Анна М.

Молекулярный биолог (Немецкий университет)

To whom it may concern,

I would like to introduce myself as a postgraduate student (master) in my last semester, studying Molecular Life Sciences at the Friedrich Schiller University Jena (after completing my undergraduate education in biochemistry at the FSU). My studies will be finished in September 2020, and then I will come back to Moscow. Currently I am completing my master’s thesis in the group of Prof. Dr. rer. nat. Baniahmad (Molecular Genetics laboratory) at the University Hospital Jena, Institute of Human Genetics. I admire studies that have an impact on the development of new therapies and improvement of people’s life quality. I would like to work as a scientist at the research institute or at the biotechnogical company in Russia.

Последнее обновление резюме 19.01.2021
Адрес Москва, Russian Federation
Электронная почта Заблокировано
Телефон Заблокировано

Опыт


University Hospital Jena, Institute of Human Genetics, Department of Molecular Genetics
Сен 2019 - Текущий
cell culture LNCaP and C4-2 cell lines, retroviral transduction, transfection (vector, siRNA), flow cytometry for cell cycle analysis, protein extraction from cells, SDS-PAGE, Western Blot, Senescence-associated beta Gal assay, Crystal violet growth assay, 3D cell culture (spheroids, tumor spheres), adhesion and migration experiments, qRT-PCR and primer design, RNA and DNA gels, restriction analysis, RNA isolation using Trifast reagent, immunofluorescence, cloning design for student, supervision of Masterstudent, practical class for students

Friedrich Schiller University Jena
Окт 2018 - Текущий
Biological faculty
Molecular Life Sciences program

Hans Knoell Institute Jena, Leibniz Institute for Natural Product Research and Infection Biology
Янв 2018 - Ноя 2018
Lab assistant

SDS-PAGE, Western Blot, BSA row on silver stained gel for protein concentration estimation, design of ELISA experiments, protein biotinylation, biotinylation verification using BLItz System, cross-linking experiments for protein dimer formation, cell culture and transfection HEK, HUVEC, recombinant protein purification on Ni-beads, Dialysis, bacterial transformation E.coli, DNA Midi-prep,
Buffer preparation

Friedrich Schiller University Jena
Окт 2016 - Сен 2018
Biological Faculty
Biochemistry major

Ruhr University Bochum
Окт 2015 - Сен 2016
Chemistry Faculty

Biochemistry major

В чем вы сильны?

Why do I think that I am a good candidate?

I completed bachelor’s degree in Biochemistry in German language and master’s degree in Molecular Life Sciences in English language. My Grade Point Average is 1.4 (4,6 in Russian system), which places me in the second position in students ranking. I managed to complete my bachelor’s education combining two universities in three years. For one year, after graduation from school in Moscow and winning a scholarship “Studienbrücke”, I have studied Biochemistry at the Chemistry Faculty at the Ruhr University Bochum that is known to provide an excellent background knowledge in chemistry. The change to the FSU Jena in my second year of undergraduate studies allowed me to study deeper molecular biology subjects, which were always my priority, e.g. biochemistry, molecular genetics and cell biology. As a result of combination of two universities, I had to take more classes and practical courses as usual. Therefore, I can say that I am ready to work hard for achievement of my goals.

I already have a real practical experience in independent work in laboratory

Aside from multiple practical classes (e.g. Center of molecular biomedicine, Biophysics department; The Leibniz Institute on Aging – Fritz Lipmann Institute, work with C.elegans, N.furzeri) and lab assistant job during my studies, I made my bachelor thesis in immunology field at Hans-Knöll Institute. During my project, I suggested my own experimental design and a new protein purification method, which were approved by my boss. Based on results of this study (ELISA was the main method), I formulated hypothesis about dual behavior of the protein of my interest in dimerization process in surface-bound- and fluid- states. Furthermore, I developed the program of experiments for the validation or rejection of this hypothesis. My boss found my project quiet prospective (given mark for bachelor thesis is 1.1, that is equal to 4.9 in Russian system).
After finishing bachelor thesis, I continued my studies in Molecular Life Science master’s program at the FSU. The master’s thesis was done at the Institute of Human Genetics (Molecular Genetics laboratory) in oncology research, where I investigated the role of protein of my interest in prostate cancer using diverse methods (3D cell culture (spheroids, tumor spheres), transfection/ retroviral transduction, Senescence associated β GAL, Western Blot, qRT-PCR, flow cytometry, immunofluorescence, scratch assay etc). At the moment, we are working on the paper containing my results. In addition, I experienced teamwork with many international colleges, giving presentations in progress reports and journal clubs, supervision of students in their practical classes at the Institute.

Расскажите о себе что-нибудь еще: публикации, конференции, хобби

Thus, I hope that my experience in working in different research institutes in field of immunology and molecular medicine, two foreign languages and enthusiasm will be suitable and useful for your research group.

Thank you for your time and consideration.

Yours faithfully,
Anna Melekhova