Designed: original flow cytometry analytical panels for ex-vivo AML theranostic assay development. Multicolor flow cytometry panel design and verification. Development of stromal cell-based culture system for LSCs research.
Training of BD Canto II, BD Accuri and BD FACSCalibur users.

Organization of the flow cytometry laboratory:
Established the new high-end level flow cytometry lab from zero-point.
Designed: original flow cytometry analytical panels and assays for anti-HIV drug and vaccine trials.
Patented: new assay/biomarker for patient immune status monitoring
Day-to-day lab and lab team management.
Multicolor (up to 12 colors) flow cytometry panel design and verification. Using of flow cytometry to monitor activation and proliferation profiles of different subsets of T cell in patient samples during multi-national clinical trials and in biomarker cell-based assay development.
Drug screening panels design and verification.
Performing the training for LSR II and Coulter Epics XL flow cytometers usage.
Providing consulting services related to flow cytometry and flow cytometry support for external collaborators (outsourcing).

Providing the expert opinion in flow cytometry and cell-based assays development fields
Providing consulting services related to flow cytometry and flow cytometry support for Biotech and Pharma clients.

Discovered: the new mechanism of iNKT functional deficiency in Type 1 diabetes
Field of study: Cell immunology. Role of invariant NKT and NK cells in the protection against Type 1 Diabetes
Experience includes: Working with murine splenic, liver, lymph nodal, bone marrow and blood samples. Cell isolation and establishing of primary cell cultures.
Cell sorting. Using multi-stage methods (FACS/MACS combination) for the sorting of rare cell subsets. Generation of mature T lymphocytes, NKT (natural killer T) and dendritic cell lines from sorted precursor cells.
Using flow cytometry for detection, immunophenotyping and cytokine profiling of T, NKT and Dendritic cells.
Using RT-PCR to evaluate genetic background of iNKT functioning and development
Performing the training for FACScalibur and FACS Canto II flow cytometers usage

Experience includes:
Using flow cytometry for detection, immunophenotyping and cytokine profiling of T, and Dendritic cells.
Performing the training for FACScan and Coulter Epics XL-MCL flow cytometers usage

Discovered: the pathway of early development of human iNKT cells in thymus and how iNKT pool is maintained in periphery.
Field of study: Cancer biology. Role of invariant NKT and NK cells in anti tumor immune responce in human neuroblastoma.
Experience includes: Working with human thymic, bone marrow and blood samples. Cell isolation and establishing of primary cell cultures.
Cell sorting. Using multi-stage methods (FACS/MACS combination) for the sorting of rare cell subsets. Generation of mature T lymphocytes, NKT (natural killer T) and dendritic cell lines from sorted precursor cells.
Using flow cytometry for detection, immunophenotyping and cytokine profiling of T and NKT cells.
Using TREC RT-PCR to evaluate the replicative history of different subsets of T and NKT cells.
Using of the mouse models and in-vivo optical imaging for evaluation of the anti-tumor effects of NKT and NK cells.