Марина П.
молекулярный биолог и начинающий биоинформатикработа на полный день
| Последнее обновление резюме | 16.05.2024 |
| Адрес | Москва / Санкт-Петербург, Russian Federation |
| Электронная почта | Заблокировано |
| Телефон | Заблокировано |
Опыт
Junior research assistant
Образование
PhD Degree
В чем вы сильны?
Molecular biology: Nucleic acid, protein, lipid, and metabolites isolation/purification and gel analysis. Dissection of various mouse organs and parts of mouse brains for RNA extraction, and following mRNA/small RNA library preparation. qRT-PCR, western blot, protein immunoprecipitation, Primer extension assay. Restriction Enzyme cloning, Megaprimer RF cloning, Gibson Assembly, TA cloning, Golden Gate cloning, Site-directed mutagenesis. Preparation of competent E.Coli cells.
Cell culture: Primary neuronal and glial cell culture. Cell lineage: use and maintenance. Transfection and transduction of mammalian cell lineages. Cell cycle analysis. Generation of stable expression mammalian cell lines. CRISPR-Cas9 gene inactivation and generation of modified cell lines.
Microscopy: Confocal and 3D light-sheet fluorescence microscopy. Immunohistochemical staining of optically cleared tissue. Transcardial perfusion. Sample sectioning. Immunohistochemistry of human and mouse brain slices from fresh specimens or frozen tissue. In situ hybridization of the snail nervous system.
Cellular biology: Single cell gel electrophoresis, metaphase and anaphase-telophase analysis of chromosomal aberrations, modified Ames test, γ-H2AX assay.
Animal manipulation: Handling, feeding, intraperitoneal injection, killing by cervical dislocation.
Scripting experience with R, Python (Pandas), and Bash. Transcriptome/Lipidome analysis. De novo transcriptome assembly.
Расскажите о себе что-нибудь еще: публикации, конференции, хобби
Petrova M., Margasyuk S., et al. BRD2 and BRD3 genes independently evolved RNA structures to control unproductive splicing. NAR Genom Bioinform. 2024.
doi.org/10.1093/nargab/lqad113
Glinin T., Petrova M., et al. Pheromone of grouped female mice impairs genome stability in male mice through stress‐mediated pathways. Scientific Reports. 2023. doi.org/10.1038/s41598-023-44647-w
Margasyuk S., Kalinina M., Petrova M., Skvortsov D., Cao C., Pervouchine D. RNA in situ confor- mation sequencing reveals novel long-range RNA structures with impact on splicing. RNA. 2023. doi.org/10.1261/rna.079508.122
Mironov A., Petrova M., Margasyuk S., Vlasenok M., Mironov A., Skvortsov D., Pervouchine D. Tissue-specific regulation of gene expression via unproductive splicing. Nucleic Acids Res. 2023. doi.org/10.1093/nar/gkad161
Daev E., Petrova M., Onopa L., Shubina V., Glinin T. DNA damage in bone marrow cells of mouse males in vivo after exposure to the pheromone: Comet assay. Russian Journal of Genetics. 2017.
Xu C., Li Q., Efimova O., Jiang X., Petrova M., Vinarskaya A., Kolosov P., Aseyev N., Koshkareva K., Ierusalimsky V., et al. Immediate early genes in the nervous system of snail Helix Lucorum. eNeuro. 2019.
Shcherbinina V., Petrova M., Glinin T., Daev E. Genotoxic effect of restraint and stress pheromone on somatic and germ cells of mouse males Mus musculus L. Ecological Genetics. 2021.
Conferenses:
Petrova M., Daev E., Glinin T., Mamontova V., Galkina S., Volnova A. Transcriptional responses in dividing cells of house mouse following pheromone stress and acute immobilization. EMBO Workshop "Proteostasis: From organelles to organisms”. 2019. (Poster)
Petrova M., Glinin T., Mamontova V., Shcherbinina V., Volnova A, Starshova P., Daev E., Khaitovich P. Molecular alterations in the brain, bone marrow, testis, and adrenal glands of stressed mice. 28th Int. "Stress and Behavior" Conference. 2020. (Oral)