-DNA extraction

-Cultivation of bacteria in liquid and agar media.

-Work with autoclave, thermostat, measuring instruments, laboratory equipment).

-Preparation of solutions

-Proficiency in basic methods of protein analysis (PAGE electrophoresis, Western blotting)

-Design and cloning of genetic constructs (ugene, snapgene, ape)

Education.

2016 – 2021
St. Petersburg, Russia
Bachelor's degree
St. Petersburg State Agrarian University (SPBGAU).
Bachelor's degree in agrochemistry and agrosoil science, profile agrochemistry and soil
science.
Graduation work: The effect of bacterial preparations on the biological activity of soddy-podzolic soil and the yield of oats in the conditions of the Leningrad Region.

2021 – 2023
St. Petersburg, Russia
Master's degree
St. Petersburg State Academic University. Zhores Ivanovich Alferov of the Russian Academy of
Sciences (SPBAU RAS)
Master's degree in physics, profile of nanobiotechnology.
Graduation work: Identification of new BRICHOS antimicrobial peptides in genomic data and their structural and functional studies

Relevant Experience.

2022 – present
St. Petersburg, Russia
Laboratory researcher
Renewable energy laboratory of SPBAU RAS
Responsibilities: Cultivation of bacteria in culture media, isolation and purification of
plasmids, induction of bacteria, isolation and chromatographic purification of proteins from
bacteria, bioinformatic analysis of RNAseq using Python/R/Linux and visualization of
proteins in PyMol and molecular docking in AlfaFold2.

2021 – 2021
St. Petersburg, Russia
Internship
Institute of Experimental Medicine of the National Medical Research Center. V. A. Almazova two-month internship.
Responsibilities: Isolation of DNA from human blood, DNA purification, PCR reaction, gel electrophoresis, work with Sanger sequencing data.

2017 – 2019
St. Petersburg, Russia
Laboratory researcher
All-Russian Scientific Research Institute of Agricultural Microbiology
Responsibilities: scientific work, chemical analysis of plants, presentations and trips to
conferences, preparation of reagents, writing articles.

Publications.

2021 Dynamics of activity of urease and rhizosphere microflora in otogeny against the
background of the use of biological products
GAMZAEVA R.S., BASHARINA M.V., SMETANIN R.V.
DOI: 10.31857/S0026364820030125
2019 Study of the structural and functional organization of arbuscular mycorrhiza in the original
and mutant lines of hop alfalfa (medicago lupulina) with different methods of inoculation
with the fungus rhizophagus irregularis
ZHELEZNYAKOV S.V., LEBEDEVA V.K., SMETANIN R.V., JAKOBI L.M.
eLIBRARY ID: https://www.elibrary.ru/item.asp?id=42807991
2019 Study of the induced mutant of hop alfalfa (medicagolupulinal. subsp. vulgariskoch) by
symbiotic traits during the formation of mycorrhizal symbiosis
L. M. JACOBI, S. V. ZHELEZNYAKOV, R. V. SMETANIN, V. K. LEBEDEVA, and A. P. KOZHEMYAKOV,
eLIBRARY ID: https://www.elibrary.ru/item.asp?id=39157063

Presentations.

2020
Moscow, Russia
Winter Shcool
Moscow Institute of Physics and Technology (MIPT)
Biological section: DNA extraction from buccal epithelial cells, PCR, gel phoresis

2020
St. Petersburg, Russia
Winter School
Alferov Federal State Institution of Higher Education and Science Saint Petersburg National
Research Academic University of the Russian Academy of Sciences (SPBAU RAS)
Expression of recombinant proteins in E. coli, purification of proteins on a chromatographic
column, dialysis, immunocytochemical staining of cancer cells, confocal microscopy

2019
St. Ptersburg, Russia
International Congress "VII Congress of the Vavilov Society of Geneticists and Breeders,
dedicated to the 100th anniversary of the Department of Genetics of St. Petersburg State
University, and associated symposiums"
St. Petersburg University (SPBU)
Study of the induced mutant of hop alfalfa (medicagolupulinal. subsp. vulgariskoch) by
symbiotic traits during the formation of mycorrhizal symbiosis.

2019
St. Petersburg, Russia
BIOCAD
Internship Scale Up
Received an offer for an internship Scale Up

Honors and Awards.

2022

Grant of the President of the Russian Federation
"Talent and Success" Foundation, Sirius
Persons who have shown outstanding abilities and have shown high achievements in a
particular field of activity

2022

St. Petersburg Government Scholarship
Competition for the right to receive nominal scholarships of the Government of St.
Petersburg for students of educational institutions of higher education

Professional Associations.

I am a member of the student council - discussion of urgent and long-term issues about university activity plans
Organization of an open day.
I am also a member of the student scientific council, established at the end of 2021, I determine the strategy for
conducting scientific intra-university conferences, I am engaged in educational scientific activities with
schoolchildren and junior students.
The senior of group.

Qualifications or Skills.

English, B2

Linux/Bash/R/Python3