Vilena I.R&D Scientist
I am searching for R&D Scientist/Postdoc position in Europe. My choice for this position is motivated by my interest in resuming work related to molecular and cell biology. I would welcome to work in the company presenting an excellent career opportunity. Job satisfaction is a critical factor to me, and working in an environment with state of the art equipment, adequately funded projects under EU-grade work standards would provide plenty of it. Being a growing company is also an important aspect for the potential in long-term opportunities. I believe that my research experience would be valuable and useful for my future career.
|Последнее обновление резюме||13.02.2018|
|Адрес||Kazan, Russian Federation|
|Соцсеть или сайт||Заблокировано|
Ph.D. in Biochemistry and Microbiology
In addition, the topography of the active centres in cardiolipin synthases was also studied. I showed that cardiolipin stabilizes the functional conformation of SecYEG dimer and is therefore mandatory for the correct folding of membrane proteins via this translocase. In the course of the research project, I obtained experience in Western Blots, 2D TLC (Two-Dimensional Thin-Layer Chromatography), SCAM (The substituted-cysteine accessibility method), and measurement of transmembrane pH gradients, maintenance of mutant libraries, transformation and P1vir phage transduction techniques.
M.Sc. in Molecular Pharmacology
To this end, I developed an ELISA protocol and was able to show that SLE patients display significantly higher immunoreactivity to total nucleic acids compared to healthy controls, as well as a differential immunoreactivity to the various nucleic acid sub-types. During my master’s studies, I also described the internalization and nuclear co-localization of anti-DNA antibodies in a cellular model.
В чем вы сильны?
English (Level 4)
Cellular biology: • Cell culture: BJ, HEK 293, MDCK, MEFs, moDC, PDC, PBMC, SPEV, NK 92-MI, Renca, LLC1. • Microbiology: Liquid and solid cultures of bacterias. • Microscopy: fluorescence and light microscopy. • Flow cytometry. • Real-time cell analysis.
Molecular biology: • PCR-based analyses: primer design, gene expression and mtDNA quantification. Transformation. P1 phage transduction.
Biochemistry: 2D-electrophoresis (SDS-PAGE, BN-PAGE and IEF) · Western blot · Fast-flow liquid chromatography of proteins (ion-exchange resin, IMAC, C18, gel-exclusion) · ELISA · Immunofluorescence ·Immunohistochemistry.
Animal skills: • Mouse handling: IP and SC injection, tail blood collection, genotyping.
Photoshop · Corel DRAW · AxioVision · Quantity One · FlowJo · GraphPad · Minitab · FACSuite · NovoExpress · JuLI STAT · RTCA
Расскажите о себе что-нибудь еще: публикации, конференции, хобби
Fellowships and awards
2012 Algarysh scholarship grant. University of Texas, Health Science Center at Houston (Texas, USA). Project: Folding and Assembly of Membrane Proteins.
2011 DAAD scholarship grant.University of Regensburg (Regensburg, Germany). Project: Interaction of IL-13 and TGF-b1 in chronic inflammation, fibrosis and carcinogenesis Inflammation-induced colon cancer in patients with inflammatory bowel disease.
I am enjoying extending my scientific knowledge, sharpen professional skills and strengthen scientific background in preparation for the future scientific career.
I am a conscientious person who works hard and pays attention to details. I'm flexible, quick to pick up new skills and eager to responsibly learn from others. I also have lots of ideas and enthusiasm